EVALUATION OF THE TOTAL POLYPHENOL CONTENT, TOTAL FLAVONOID CONTENT, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF BOESENBERGIA PANDURATA RHIZOME EXTRACT
Abstract
This study investigated the total polyphenol content (TPC) and total flavonoid content (TFC) as well as evaluated the antioxidant and antibacterial activities of the methanol extract of the Boesenbergia pandurata rhizomes. The results demonstrated that the methanol was rich in total polyphenol content and total flavonoid content, with the TPC and TFC values of 191.44 ± 5.66 mg GAE/g extract and 121.23 ± 3.82 mg QE/g extract, respectively. Furthermore, this extract also exhibited good antioxidant activity via the DPPH assay with the IC50 value of 36.02 ± 0.13 mg/mL and it had strong antibacterial activity against Gram-positive strains, S. aureus, B. subtilis, and L. fermentum with the IC50 values of 6.63 ± 0.41, 5.83 ± 0.35, and 9.18 ± 0.51 mg/mL, respectively. However, it was inactive with the Gram-negative strains, S. enterica, E. coli, and P. aeruginosa. Our findings suggest that B. pandurata rhizomes could be a promising source of the antioxidant and antibacterial activities.
Tài liệu tham khảo
[1] Y. Wang et al., “Traditional usages, chemical metabolites, pharmacological activities, and pharmacokinetics of Boesenbergia rotunda (L.) Mansf.: a comprehensive review,” 2025. doi: 10.3389/fphar.2025.1527210.
[2] T. Eng-Chong et al., “Boesenbergia rotunda: From ethnomedicine to drug discovery,” 2012. doi: 10.1155/2012/473637.
[3] Vu Ngoc Toan et al., “Isolation and structure determination of flavonoids from the rhizomes of Boesenbergia pandurata Roxb,” J. Mil. Sci. Technol., 2025, doi: 10.54939/1859-1043.j.mst.imbe.2025.50-55.
[4] H. X. Nguyen, P. Q. Le, M. T. T. Nguyen, and N. T. Nguyen, “Flavanones from the rhizomes of Boesenbergia pandurata,” Sci. Technol. Dev. J. - Nat. Sci., 2019, doi: 10.32508/stdjns.v2i4.811.
[5] N. T. Nguyen et al., “Constituents of the rhizomes of Boesenbergia pandurata and their antiausterity activities against the PANC-1 human pancreatic cancer line,” J. Nat. Prod., 2017, doi: 10.1021/acs.jnatprod.6b00784.
[6] T. L. E. Nguyễn, T. L. H. Nguyễn, D. K. Trần, T. M. H. Nguyễn, V. V. Phạm, and H. P. Ngô, “Khảo sát sơ bộ đặc điểm thực vật học, thành phần hóa học của cây ngải bún (Boesenbergia pandurata (roxb.) schltr.) thu hái tại tỉnh Sóc trăng,” Tạp chí Y Dược học Cần Thơ, 2024, doi: 10.58490/ctump.2024i81.2866.
[7] O. Ongwisespaiboon and W. Jiraungkoorskul, “Fingerroot, Boesenbergia rotunda and its aphrodisiac activity,” 2017. doi: 10.4103/phrev.phrev_50_16.
[8] R. Lucas-González, M. Pateiro, R. Domínguez-Valencia, C. Carrillo, and J. M. Lorenzo, “Optimization of Anthocyanin Extraction from Purple Sweet Potato Peel (Ipomea batata) Using Sonotrode Ultrasound-Assisted Extraction,” Foods, 2025, doi: 10.3390/foods14152686.
[9] S. Thummajitsakul et al., “Total phenolic content, antioxidant and antibacterial activities of extracts from Alpinia and Boesenbergia genera,” J. Appl. Biol. Chem., 2024, doi: 10.3839/jabc.2024.043.
[10] F. I. Fahrudin, S. Phongthai, T. I. Wirjantoro, and P. Intipunya, “Synergistic Effects of Pressure, Temperature, CO2 Flow Rate and Co-Solvent on Bioactive Contents of Thai Fingerroot (Boesenbergia rotunda (L.) Mansf.) Extracts,” Foods, 2025, doi: 10.3390/foods14132189.
[11] N. Liwanda, Z. K. Nisa, S. Windrianti, T. A. Soplanit, and W. Nurcholis, “Solvent optimization enhanced phenolic content and antioxidant activity in ultrasound-assisted extraction of Boesenbergia rotunda rhizomes,” Int. J. Agric. Technol., 2025.
[12] J. J. Lee, J. Choi, and J. H. Ha, “Physicochemical and Storage Characteristics of Pork Tteokgalbi Treated with Boesenbergia pandurata (Roxb.) Powder,” Appl. Sci., 2022, doi: 10.3390/app12052425.
[13] S. Thomya, M. Wongkaew, W. Bundithya, C. Lumsangkul, P. Jantrawut, and S. R. Sommano, “Relationship between Phenotypes and Chemical Profiling of Boesenbergia rotunda Collected from Different Habitats of Northern Thailand,” Agronomy, 2023, doi: 10.3390/agronomy13061435.
[14] D. H. Truong, D. H. Nguyen, N. T. A. Ta, A. V. Bui, T. H. Do, and H. C. Nguyen, “Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia,” J. Food Qual., 2019, doi: 10.1155/2019/8178294.
[15] İ. Gulcin and S. H. Alwasel, “DPPH Radical Scavenging Assay,” 2023. doi: 10.3390/pr11082248.
[16] P. Rithichai, Y. Jirakiattikul, P. Poljan, N. Youngvises, and A. Itharat, “Growth, bioactive compound accumulation and antioxidant activity in rhizomes and storage roots of Boesenbergia rotunda (L.) Mansf.,” Agric. Nat. Resour., 2022, doi: 10.34044/j.anres.2022.56.2.08.
[17] F. I. Fahrudin, P. Intipunya, S. Phongthai, and T. I. Wirjantoro, “Screening of Antioxidant Activity, Total Phenolics, and Flavonoid on Selected Northern Thailand Medicinal Rhizomes,” 2024. doi: 10.2991/978-94-6463-451-8_21.
[18] A. Zeb, “Concept, mechanism, and applications of phenolic antioxidants in foods,” 2020. doi: 10.1111/jfbc.13394.
[19] D. M. Pereira, P. Valentão, J. A. Pereira, and P. B. Andrade, “Phenolics: From chemistry to biology,” Molecules, 2009, doi: 10.3390/molecules14062202.
[20] R. Barbieri et al., “Phytochemicals for human disease: An update on plant-derived compounds antibacterial activity,” 2017. doi: 10.1016/j.micres.2016.12.003.